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CrossProfilesMacro for Coli-Inspector

Norbert Vischer
18-Feb-2019

This macro is intended for cells that have already been analyzed with Coli-Inspector.

(See also the CrossProfiles.ojj project for manual marking)

It qualitatively analyses the ratio between membrane- vs. cytoplasm location of fluorescent proteins by creating intensity profiles perpendicuar to the cell axes.

Download CrossProfilesMacro

Measurement steps:

• open your Coli-Inspector.ojj project file containing marked cells

• Choose ImageJ>File>Open.., and open the CrossProfileMacro1.x.txt

• Choose menu Macros>Run Macro

After execution, three profile plots are created:

• One Peak
  
• Two Peaks
  
• OneP+TwoP (a weighted sum of the above)
  

When you definitely expect a 2-peak profile , take the data from the Two-Peak profile (column CB2-Ratio) which is not spoilt by mainly artefacts that created one peak.

Make sure to use the newest versions of ImageJ and ObjectJ.

Fig. 1 Evaluation areas across the cell

Fig. 2 Linked Results show CB1-Ratio, CB2Ratio, and CB-Ratio

Fig. 3 Three collective plots of all profiles