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Coli-Inspector News

version 04g (01-apr-2022)

'Rebuild Map' could show gaps or errors in 'Diameter Map' for linked images that were saved with a ROI. This is fixed.

version 04f (18-mar-2022)

Menu 'Subtract Fluor. Background' compatible with ImageJ 1.53p (which dropped the "Table..." command)

version 04e (23-mar-2021)

Help button of Collective Profiles
flag intFluorL and intFluorT in embedded macros
to distinguish between mean and integrated fluorescence in longitudinal(L) and transversal(T) direction in Maps and Plots

version 04d (30-aug-2020)

fixed bug for processing non-stacks

version 04c (14-jul-2020)

removed menu "Create Demo-Stack"
included Help button in "Update Map-depending results"
appended "ObjectJ>Help" menu

version 04a (18-may-2020)

adjustable resolution of Collective Profiles via variable "nProfilePoints"

version 03z (03-apr-2020)

showing images too small and at bottom: this should be fixed with newer ImageJ versions
speed "Mark Filaments" improvement (2000 cells/min)

version 03v (22-jul-2019)

Included Contour item to show cell contour permanently.
Position of constriction is interpolated and more precise
Renamed spots to BSpot,CSpot, DSpot for easier channel annotation (chn 2,3,4, respectively )

version 03s (08-mar-2018)

Included command ObjectJ>Navigate>Reverse Axis[R], which puts the start point of the cell axis (indicated by the number label) to the opposite side.

version 03r (19-dec-2017)

Global variable "extendLength" can be used to extend the profile to catch fluorescece beyond either cell pole. It is defined as extra pixels on either side.
global variable endPiece can be used to define pole (default = 0.6 um),

version 03q (14-dec-2017)

Due to a regression in ImageJ (versions 1.51r26 to 1.51t11), analysis stopped after the second linked image.
Either update to the newest daily build of ImageJ, or use the newest Coli-Inspector (03q) which includes a work-around....

version 03p (30-aug-2016)

Subtract fluor. background could be confused (showing values of 65535)
in overexposed 16-bit images. This is fixed.

version 03o (17-aug-2016)

'Navigate> Zoom to next/previous object' will close the oldest
open and unchanged image if less than 200 MB memory is available

Graph-Assistant (06-mar-2016)

Error bars did not work with new ImageJ version > 1.48

version 03n (26-aug-2015)

Fixed a bug that could show zero volume in noisy images

version 03m (31-may-2015)

Subtract Background works for multi-frames or multi-slices,

version 03k (26-may-2015)

Histogram during background subtraction not asking for stack/slice,

version 03j (30-sep-2014)

Added option for manual Y-Range in Collective Profiles

version 03i (25 aug-2014)

Added option to disable normalisation of collective profiles

version 03h (22-aug-2014)

Added area column

version 03f (10-jul-2014)

Constrictions are marked with correct angle (cosmetic)

version 03e (08-jul-2014)

"Collective Profile" creates additional results table if number of AgeGroups > 1
fixed a bug that said "Map was not found"
fixed a bug that blocked analysis of single-channel stacks

version 03c (08-may-2014)

Fixed a bug which showed "Collective Profile" unflipped although "Leader Channel for Flipping" was active

version 03b (10-apr-2014)

Fixed a bug which didn't allow single-channel (PhC only) profile maps

version 03a (14-mar-2014)

Fixed a bug which suppressed leader channel in scatter plots
Scatter plots can now used mixed markers
Scatter plots have "Symmetrical Plots" checkbox: if checked, each spot appears twice in the plot (normal and mirrored).

version 02z Beta!(14-mar-2014)

Reorganized order of macro commands
Detection of multiple constrictions
"Collective Profiles" dialog has a "Symmetrical Plots" checkbox
Scatter plot spot positions can optionally be flipped to follow leader channel
Evaluation of leader pole can be adjusted to max 0.5 of cell length
Disabled advanced spot columns (calcavgOffsets = false)
Included calculation of "Age" columm; longest cell correctly shows 100%

version 02y (12-mar-2014)

Internally reorganized to match with ChainTracer-no functional difference
Fixed a bug that resulted in lost spot offsets in static column _OffC

version 02x (04-mar-2014)

Collective profiles can be resolved in age groups.

version 02w (21-feb-2014)

Subtract Fluor. Backgrounds presents an editable table of background values before performing subtraction.

version 02v (04-feb-2014)

Fixed a bug (introduced in version 02s) which after deleting cells left some of the lowest result fields empty (non-empty fields were correct).
Fixed a bug that could decrease the "MaxDia" condition when "Mark filaments" was run again after "Update Map-dependent Results".

version 02s (27-jan-2014)

"Subtract Fluor Background.." visualizes background numerically and graphically

version --- (24-jan-2014)

Solved "Jolanda" bug better

version --- (23-jan-2014)

Faster Marking and faster Rebuild Map
Solved "Jolanda" bug: "could not save because Result too large"
Process is less senistive to inadverted user clicks during processing
Show Outlines works faster; command renamed to Show/Hide Outlines [F6]
Added a IntFluorFlag: if true, profiles contain integrated fluorescence instead mean

version 02q (7-jan-2014)

command Advance [A] was not working- this bug is fixed
Simplified menus (set advanced=true, and uncomment tail of embedded macros to re-activate special features)
Fixed a bug that caused Rebuild Map not working when one of the stacks was saved in Color mode = Composite.
updated website

versions 02e .. 02p (oct..dec-2013)

many undocumented improvements,
spot items are now strictly bound to channels depending on their appearance in the ObjectJ tools, starting at channel 2.

version 02c (6-oct-2013)

fixed a bug introduced at version 01u (july 2013). Due to this bug, the fluorescence profile was evaluated with a too thick line width. Thus for long cells with close neighbors, possibly some foreign fluorescence was added. Repair instructions:
```
check version via ObjectJ>Show Embedded Macros (top lines)
if version is 01u .. 02b, your data may be affected, then:  
update and install embedded macros
run Object>Rebuild Map
run Object>Update Map-dependent Results  
```